Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2003 Aug;5(8):701-10.
doi: 10.1038/ncb1020.

Cdk5 is essential for synaptic vesicle endocytosis

Timothy C Tan  1 , Valentina A Valova, Chandra S Malladi, Mark E Graham, Leise A Berven, Orla J Jupp, Gurdip Hansra, Sonya J McClure, Boris Sarcevic, Ross A Boadle, Martin R Larsen, Michael A Cousin, Phillip J Robinson
Affiliations

Cdk5 is essential for synaptic vesicle endocytosis

Timothy C Tan et al. Nat Cell Biol. 2003 Aug.

Abstract

Synaptic vesicle endocytosis (SVE) is triggered by calcineurin-mediated dephosphorylation of the dephosphin proteins. SVE is maintained by the subsequent rephosphorylation of the dephosphins by unidentified protein kinases. Here, we show that cyclin-dependent kinase 5 (Cdk5) phosphorylates dynamin I on Ser 774 and Ser 778 in vitro, which are identical to its endogenous phosphorylation sites in vivo. Cdk5 antagonists and expression of dominant-negative Cdk5 block phosphorylation of dynamin I, but not of amphiphysin or AP180, in nerve terminals and inhibit SVE. Thus Cdk5 has an essential role in SVE and is the first dephosphin kinase identified in nerve terminals.

PubMed Disclaimer

Comment in

  • Cdk5 is a dynamo at the synapse.
    Samuels BA, Tsai LH. Samuels BA, et al. Nat Cell Biol. 2003 Aug;5(8):689-90. doi: 10.1038/ncb0803-689. Nat Cell Biol. 2003. PMID: 12894171 No abstract available.

Publication types

MeSH terms