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. 2003;37(2):115-20.
doi: 10.1046/j.1472-765x.2003.01360.x.

Development of an inducible gene expression system for Lactobacillus sakei

L Axelsson  1 , G Lindstad, K Naterstad
Affiliations

Development of an inducible gene expression system for Lactobacillus sakei

L Axelsson et al. Lett Appl Microbiol. 2003.

Abstract

Aim: To develop an inducible gene expression system for Lactobacillus sakei, based on the regulatory system of sakacin A production.

Methods and results: A Lactobacillus/Escherichia coli shuttle vector; pKRV3, was constructed including the signal transducing system genes of the bacteriocin sakacin A. The gusA gene fused to PsapA promoter, cloned in this vector allowed for inducible beta-glucuronidase expression in L. sakei and L. plantarum following the addition of the sakacin A inducing peptide. PsapA appeared to be a strong and tightly controlled promoter when compared with known promoters.

Conclusion: The pKRV3 system can be used as an inducible gene expression system in lactobacilli.

Significance and impact of the study: A novel, inducible gene expression system has been developed for lactic acid bacteria relevant in food fermentations.

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