Dentinal composition and Knoop hardness measurements of cavity floor following carious dentin removal with Carisolv

Abstract

This study evaluated the dentinal composition and Knoop hardness measurements of the cavity floor following the removal of carious dentin by the Carisolv chemo-mechanical caries removal system, in vitro. The carious dentin of 25 extracted human teeth was removed by using Carisolv for one minute with instruments and excavation that was performed until the gel was clear. Caries removal with a sharp explorer was verified according to the color and hardness of the lesion, then, by means of DIAGNOdent. Atomic analysis of treated cavities was performed by energy dispersive x-ray spectroscopy (SEM-EDX) and the Knoop hardness number (KHN) of the cavity floor was determined. Surface characteristics were observed by the scanning electron microscope (SEM). Adjacent sound dentin was used as a control reference. No significant differences were found between the quantities of calcium content (Ca weight %), phosphorus content (P weight %) and the Ca/P weight ratio of Carisolv cavities with that of the adjacent, sound dentin (p<0.01). KHN of the Carisolv cavity floor was almost similar to that of the adjacent sound dentin. SEM analysis revealed an extremely rough or irregular surface, and there remained a minimal debris-like smear layer; most of the dentinal tubules were opened. The results indicated that Carisolv does not produce any adverse side effects on dentinal compositions of the treated cavities. The possibility of remaining residual softened dentin was also minimal in this study.