Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2003 Jul 31;46(16):3448-51.
doi: 10.1021/jm0340896.

Effect of detergent on "promiscuous" inhibitors

Ali J Ryan  1 , Norman M Gray, Peter N Lowe, Chun-wa Chung
Affiliations

Effect of detergent on "promiscuous" inhibitors

Ali J Ryan et al. J Med Chem. .

Abstract

The term "promiscuous" inhibitors has been coined for compounds whose inhibition mechanism involves the interaction of aggregates of many compound molecules with the target protein, rather than the binding of individual molecules. This paper demonstrates that promiscuous inhibitors can be differentiated from classical 1:1 inhibitors by the judicious use of detergents, making it possible to configure assays that significantly reduce this undesirable mechanism of inhibition without compromising assay performance.

PubMed Disclaimer

LinkOut - more resources