[Multiplicity of beta lactamases: a problem of isoenzymes]

Abstract

Analytical isoelectric focusing of beta lactamases is a very sensitive and powerful technique which generally shows a number of satellites near the main enzymatic spike. By this technique we studied two bacterial groups known to produce different beta lactamases, but only one by germ. Analytical isoelectric focusing showed that all bacteria from the same group produce the same satellite system which differs only in relative activity.