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Comparative Study
. 2003 Jan;25(1):51-4.
doi: 10.1023/a:1021730131633.

Isolation and biochemical characterization of a new NADH oxidase from Lactobacillus brevis

Werner Hummel  1 , Bettina Riebel
Affiliations
Comparative Study

Isolation and biochemical characterization of a new NADH oxidase from Lactobacillus brevis

Werner Hummel et al. Biotechnol Lett. 2003 Jan.

Abstract

A new NADH oxidase, useful for the regeneration of NAD+, was isolated and characterized from Lactobacillus brevis. In crude extracts the activity was from 10-15 U mg(-1). After purification by four chromatographic steps, an activity of 116 U mg(-1) was obtained with 14% yield. Highest activity was from pH 5.5-7 and at 40 degrees C. The enzyme requires dithiothreitol to prevent oxidative deactivation. The Km value for NADH was 24 microM.

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