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. 2003;21(2):93-5.

[Construction and expression of the recombinant plasmid pcDNA3.1/Ts87 of Trichinella spiralis]

[Article in Chinese]
Ya-ping Yang  1 , Xin-ping Zhu, Jing Yang, Lu-ping Zhang, Xu-na Xu, Song Huang, Pascal Boireau
Affiliations
  • PMID: 12884619

[Construction and expression of the recombinant plasmid pcDNA3.1/Ts87 of Trichinella spiralis]

[Article in Chinese]
Ya-ping Yang et al. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2003.

Abstract

Objective: To construct and express DNA plasmid of Trichinella spiralis.

Methods: The BamHI and HindIII enzyme sites and KOZAK sequences were introduced at both ends of Ts87 gene by PCR. Ts87 gene was ligated into pcDNA3.1(+) vector with T4 ligase. The recombinant plasmid pcDNA3.1/Ts87 and plasmid pcDNA3.1 were transfected into an eukaryotic cell line COS7 through Lipofectamine, respectively. The BALB/c mice were immunized with the purified plasmid DNA pcDNA3.1/Ts87 through two routes: intramuscular injection and gene-gun injection.

Results and conclusion: The pcDNA3.1/Ts87 was expressed both in COS7 and in the BALB/c mice.

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