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. 1992 Nov 15;201(10):1549-52.

Production, characterization, and applications of a murine monoclonal antibody to dog erythrocyte antigen 1.1

G A Andrews  1 , P S Chavey, J E Smith
Affiliations
  • PMID: 1289333
Free article

Production, characterization, and applications of a murine monoclonal antibody to dog erythrocyte antigen 1.1

G A Andrews et al. J Am Vet Med Assoc. .
Free article

Abstract

A murine IgM monoclonal antibody, which recognizes dog erythrocyte antigen (DEA) 1.1, has been produced. The antibody correctly identified canine RBC possessing DEA 1.1 in a panel of RBC typed by an independent laboratory. Reactivity of the monoclonal antibody was compared with canine anti-DEA 1.1 antiserum with 163 RBC samples from 145 dogs. Results of agglutination tests with the 2 reagents were in agreement for all samples. A card agglutination test that uses the monoclonal antibody with blood is described. A monoclonal antibody-based test should facilitate blood typing for DEA 1.1 in clinical practice.

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