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. 2003 Aug;69(8):5001-5.
doi: 10.1128/AEM.69.8.5001-5005.2003.

Characterization of Anaplasma marginale isolated from North American bison

José De La Fuente  1 , Elizabeth J Golsteyn Thomas, Ronald A van den Bussche, Robert G Hamilton, Elaine E Tanaka, Susan E Druhan, Katherine M Kocan
Affiliations

Characterization of Anaplasma marginale isolated from North American bison

José De La Fuente et al. Appl Environ Microbiol. 2003 Aug.

Abstract

Anaplasma marginale (Rickettsiales: Anaplasmataceae), a tick-borne pathogen of cattle, is endemic in tropical and subtropical regions of the world. Although serologic tests have identified American bison, Bison bison, as being infected with A. marginale, the present study was undertaken to confirm A. marginale infection and to characterize isolates obtained from naturally infected bison in the United States and Canada. Major surface protein (MSP1a and MSP4) sequences of bison isolates were characterized in comparison with New World cattle isolates. Blood from one U.S. bison was inoculated into a susceptible, splenectomized calf, which developed acute anaplasmosis, demonstrating infectivity of this A. marginale bison isolate for cattle. The results of this study showed that these A. marginale isolates obtained from bison were similar to ones from naturally infected cattle.

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Figures

FIG. 1.
FIG. 1.
Phylogenetic affinities among isolates of A. marginale based on unweighted maximum parsimony analysis of character state changes of msp4. Topology of 50% majority rule consensus tree of 59 equally parsimonious trees of 193 steps (consistency index = 0.7629; retention index = 0.9205) is shown. Numbers above branches are percentages of 500 bootstrap iterations in which each clade was detected.
FIG. 2.
FIG. 2.
Structure of the MSP1a repeat region for bison and U.S. cattle isolates of A. marginale using the repeat forms in Table 3.
See this image and copyright information in PMC

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