doi: 10.1093/nass/2.1.77.
tRNA recognition by CCA-adding enzyme
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- PMID: 12903113
- DOI: 10.1093/nass/2.1.77
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tRNA recognition by CCA-adding enzyme
Nucleic Acids Res Suppl.
2002.
Abstract
The CCA-adding enzyme [ATP (CTP): tRNA nucleotidyltransferase (EC. 2.7.7.25)] catalyzes the addition of the conserved CCA sequence to the 3'-terminus of tRNAs. It is known that CCA-adding enzymes from all three kingdoms recognize the elbow region of L-shape tRNA formed by the tertiary interaction between T-loop and D-loop. We here demonstrate that C. elegans mitochondrial CCA-adding enzyme has broad substrate specificity toward nematode mitochondrial tRNAs which lack entire T- or D-arms, by cloning and characterizing the recombinant protein.
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