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. 2002:(2):77-8.
doi: 10.1093/nass/2.1.77.

tRNA recognition by CCA-adding enzyme

Yukihide Tomari  1 , Tsutomu Suzuki, Takuya Ueda
Affiliations

tRNA recognition by CCA-adding enzyme

Yukihide Tomari et al. Nucleic Acids Res Suppl. 2002.

Abstract

The CCA-adding enzyme [ATP (CTP): tRNA nucleotidyltransferase (EC. 2.7.7.25)] catalyzes the addition of the conserved CCA sequence to the 3'-terminus of tRNAs. It is known that CCA-adding enzymes from all three kingdoms recognize the elbow region of L-shape tRNA formed by the tertiary interaction between T-loop and D-loop. We here demonstrate that C. elegans mitochondrial CCA-adding enzyme has broad substrate specificity toward nematode mitochondrial tRNAs which lack entire T- or D-arms, by cloning and characterizing the recombinant protein.

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