doi: 10.1093/nass/2.1.233.
Trans-kingdom conjugation in vitro by using toluenized cells
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- PMID: 12903191
- DOI: 10.1093/nass/2.1.233
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Trans-kingdom conjugation in vitro by using toluenized cells
Nucleic Acids Res Suppl.
2002.
Abstract
To establish in vitro trans-kingdom conjugation, we developed a novel direct cell PCR by using toluenized cells which permeate enzymes, primers and substrates. The cells was shown to be successful recipients in conjugation.
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