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. 2002:(2):233-4.
doi: 10.1093/nass/2.1.233.

Trans-kingdom conjugation in vitro by using toluenized cells

Riko Tonomoto  1 , Hiroyuki Sonoda, Katsunori Suzuki, Kazuo Yoshida
Affiliations

Trans-kingdom conjugation in vitro by using toluenized cells

Riko Tonomoto et al. Nucleic Acids Res Suppl. 2002.

Abstract

To establish in vitro trans-kingdom conjugation, we developed a novel direct cell PCR by using toluenized cells which permeate enzymes, primers and substrates. The cells was shown to be successful recipients in conjugation.

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