doi: 10.1093/nass/44.1.121.
Synthesis of a thymine glycol building block and its incorporation into oligonucleotides
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- PMID: 12903298
- DOI: 10.1093/nass/44.1.121
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Synthesis of a thymine glycol building block and its incorporation into oligonucleotides
Nucleic Acids Symp Ser.
2000.
Abstract
A phosphoramidite building block of thymine glycol, a major oxidative base damage in DNA, was synthesized in four steps from protected thymidine. The two hydroxyl functions of the oxidized base were protected with the tert-butyldimethylsilyl group. Using this building block, oligonucleotides containing this damage were synthesized.
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