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. 2003 Aug;41(8):3870-2.
doi: 10.1128/JCM.41.8.3870-3872.2003.

Quantitative real-time PCR assay for detection of Ehrlichia chaffeensis

Amanda D Loftis  1 , Robert F Massung, Michael L Levin
Affiliations

Quantitative real-time PCR assay for detection of Ehrlichia chaffeensis

Amanda D Loftis et al. J Clin Microbiol. 2003 Aug.

Abstract

A real-time PCR assay was developed for the detection of Ehrlichia chaffeensis. The assay is species specific and provides quantitative results in the range 10 to 10(10) gene copies. The assay is not inhibited by the presence of tick, human, or mouse DNA and is compatible with high sample throughput. The assay was compared with previously described assays for E. chaffeensis.

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