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. 2003 Aug;41(8):3893-6.
doi: 10.1128/JCM.41.8.3893-3896.2003.

VIM-1 Metallo-beta-lactamase-producing Klebsiella pneumoniae strains in Greek hospitals

P Giakkoupi  1 , A Xanthaki, M Kanelopoulou, A Vlahaki, V Miriagou, S Kontou, E Papafraggas, H Malamou-Lada, L S Tzouvelekis, N J Legakis, A C Vatopoulos
Affiliations

VIM-1 Metallo-beta-lactamase-producing Klebsiella pneumoniae strains in Greek hospitals

P Giakkoupi et al. J Clin Microbiol. 2003 Aug.

Abstract

Seventeen Klebsiella pneumoniae clinical isolates carrying the bla(VIM-1) metallo-beta-lactamase gene were collected in the intensive care units of three hospitals in Athens, Greece, in 2002. They exhibited various carbapenem resistance levels (Etest MICs of imipenem ranged from 4 to 32 microg/ml). All isolates gave positive results by the imipenem-EDTA synergy Etest. The isolates were classified into four main types by pulsed-field gel electrophoresis; the majority of the isolates (5 and 10 isolates) belonged to two types. The bla(VIM-1) gene cassette was part of the variable region of a class 1 integron that also included aac6, dhfrI, and aadA. This structure was carried by transferable plasmids.

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Figures

FIG. 1.
FIG. 1.
PFGE patterns of K. pneumoniae isolates carrying VIM-type MBL genes. Lanes 1 and 2, isolates Kp1 and Kp2 (pattern A); lane 3, isolate Kp5 (pattern B); lane 4, isolate Kp6 (pattern A); lanes 5 and 6, isolates Kp7 and Kp8 (pattern C); lane 7, isolate Kp11 (pattern C1); lane 8, isolate Kp12 (pattern D); lanes 9 and 10, isolates Kp13 and Kp16 (pattern C).
FIG. 2.
FIG. 2.
(A) PstI restriction profiles of the blaVIM-1-carrying plasmids extracted from E. coli transconjugants trc-1, trc-2 (plasmid type 1), trc-8, and trc-13 (plasmid type 2) are presented in lanes 1 to 4, respectively. (B) Hybridization of the preparations shown in panel A with a blaVIM-1 probe.
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