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Case Reports
. 2003 Aug;41(8):3973-7.
doi: 10.1128/JCM.41.8.3973-3977.2003.

Seronegative bacteremic melioidosis caused by Burkholderia pseudomallei with ambiguous biochemical profile: clinical importance of accurate identification by 16S rRNA gene and groEL gene sequencing

Patrick C Y Woo  1 , Susanna K P Lau, Gibson K S Woo, Ami M Y Fung, Antonio H Y Ngan, Wai-Ting Hui, Kwok-Yung Yuen
Affiliations
Case Reports

Seronegative bacteremic melioidosis caused by Burkholderia pseudomallei with ambiguous biochemical profile: clinical importance of accurate identification by 16S rRNA gene and groEL gene sequencing

Patrick C Y Woo et al. J Clin Microbiol. 2003 Aug.

Abstract

An aerobic gram-negative bacterium was isolated from the blood and sputum of an 84-year-old, chair-bound nursing home resident with acute bacteremic pneumonia. Although the phenotypic characteristics suggested that the bacterium could be Burkholderia pseudomallei, the Vitek 1 system (GNI+), which can successfully identify 99% of B. pseudomallei strains, showed that the bacterium was "unidentified." Immunoglobulin G against the lipopolysaccharide (LPS) of B. pseudomallei, as detected by an LPS-based enzyme-linked immunosorbent assay with 95% sensitivity, was negative in both the acute-phase and convalescent-phase sera. Sequencing of the groEL gene showed that the isolate was B. pseudomallei. Proper identification of the bacterium in this study is crucial, since there would be a radical difference in the duration of antimicrobial therapy.

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Figures

FIG. 1.
FIG. 1.
Phylogenetic tree showing the relationship of the blood culture isolate to other Burkholderia species. The tree was inferred from 16S rRNA sequence data by the neighbor-joining method. A total of 1,308 nucleotide positions were included in the analysis. The tree was rooted with the sequence of B. pertussis. Bootstrap values were calculated from 1,000 trees. The scale bar indicates the number of substitutions per 100 bases using the Jukes-Cantor correction. Names and accession numbers are given as cited in the GenBank database.
FIG. 2.
FIG. 2.
Phylogenetic tree showing the relationship of the blood culture isolate to other Burkholderia species. The tree was inferred from groEL sequence data by the neighbor-joining method. A total of 1,618 nucleotide positions were included in the analysis. The tree was rooted with the sequence of B. pertussis. Bootstrap values were calculated from 1,000 trees. The scale bar indicates the number of substitutions per 100 bases using the Jukes-Cantor correction. Names and accession numbers are given as cited in the GenBank database.
See this image and copyright information in PMC

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