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. 2003 Jul;110(1):53-60.
doi: 10.1385/abab:110:1:53.

Calcium alginate gel as encapsulation matrix for coimmobilized enzyme systems

A Blandino  1 , M Macías, D Cantero
Affiliations

Calcium alginate gel as encapsulation matrix for coimmobilized enzyme systems

A Blandino et al. Appl Biochem Biotechnol. 2003 Jul.

Abstract

Encapsulation within calcium alginate gel capsules was used to produce a coimmobilized enzyme system. Glucose oxidase (GOD) and catalase (CAT) were chosen as model enzymes. The same values of Vmax and Km app for the GOD encapsulated system and for the GOD-CAT coencapsulated system were calculated. When gel beads and capsules were compared, the same catalyst deactivation sequence for the two enzymes was observed. However, when capsules were employed as immobilization support, GOD efficiencies were higher than for the gel beads. These results were explained in terms of the structure of the capsules.

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