Increased hepatic apoptosis during short-term caloric restriction is not associated with an enhancement in caspase levels
- PMID: 12915211
- DOI: 10.1016/s0531-5565(03)00091-3
Increased hepatic apoptosis during short-term caloric restriction is not associated with an enhancement in caspase levels
Abstract
Long-term caloric restriction extends lifespan, probably through a reduction in radical production and attenuation of oxidative stress. In addition, caloric restriction is associated with a reduction and incidence in tumor pathology, probably, in part, via an enhanced rate of apoptosis. We examined whether short-term (2-month) caloric restriction (40% reduction compared to ad libitum controls) increased hepatic apoptosis and if this was associated by an enhancement in various proteolytic caspase (-3, -7, -9, -12) levels and/or a decrease in two potential inhibitors of apoptosis (the x-linked inhibitor of apoptosis protein XIAP and heat shock protein 70). Short-term caloric restriction resulted in a significant decline, compared to ad libitum controls, in both body mass (30%) and liver mass (46%). While hepatic apoptosis (DNA fragmentation) was significantly higher in the caloric restricted rats, this was not associated with any increase in caspase (-3, -7, -9, -12) levels in the liver. Indeed, the levels of caspase-3, -7 and -12 were significantly lower in the caloric restricted group compared to the ad libitum controls and no differences were observed between groups in either XIAP or HSP70 levels. These findings suggest that enhanced hepatic apoptosis observed after 2-months of caloric restriction is not a result of elevated caspase levels at this time, thereby suggesting that an alternative, caspase-independent pathway may be involved.
Similar articles
-
Short-term caloric restriction and regulatory proteins of apoptosis in heart, skeletal muscle and kidney of Fischer 344 rats.Biogerontology. 2003;4(3):141-7. doi: 10.1023/a:1024149923693. Biogerontology. 2003. PMID: 12815313
-
Aging and lifelong calorie restriction result in adaptations of skeletal muscle apoptosis repressor, apoptosis-inducing factor, X-linked inhibitor of apoptosis, caspase-3, and caspase-12.Free Radic Biol Med. 2004 Jan 1;36(1):27-39. doi: 10.1016/j.freeradbiomed.2003.10.003. Free Radic Biol Med. 2004. PMID: 14732288
-
Effects of age and caloric restriction on brain neuronal cell death/survival.Ann N Y Acad Sci. 2004 Jun;1019:96-105. doi: 10.1196/annals.1297.018. Ann N Y Acad Sci. 2004. PMID: 15247000
-
Effect of chronic caloric restriction on hepatic enzymes of intermediary metabolism in the male Fischer 344 rat.Mech Ageing Dev. 1989 May;48(2):179-89. doi: 10.1016/0047-6374(89)90049-3. Mech Ageing Dev. 1989. PMID: 2661933 Review.
-
Effects of aging and caloric restriction on hepatic drug metabolizing enzymes in the Fischer 344 rat. I: The cytochrome P-450 dependent monooxygenase system.Mech Ageing Dev. 1989 May;48(2):145-55. doi: 10.1016/0047-6374(89)90046-8. Mech Ageing Dev. 1989. PMID: 2661932 Review.
Cited by
-
The effect of calorie restriction on the presence of apoptotic ovarian cells in normal wild type mice and low-plasma-IGF-1 Laron dwarf mice.J Ovarian Res. 2013 Sep 24;6(1):67. doi: 10.1186/1757-2215-6-67. J Ovarian Res. 2013. PMID: 24063422 Free PMC article.
-
Feed Restriction Alleviates Chronic Thermal Stress-Induced Liver Oxidation and Damages via Reducing Lipid Accumulation in Channel Catfish (Ictalurus punctatus).Antioxidants (Basel). 2022 May 17;11(5):980. doi: 10.3390/antiox11050980. Antioxidants (Basel). 2022. PMID: 35624844 Free PMC article.
-
Caloric restriction shortens lifespan through an increase in lipid peroxidation, inflammation and apoptosis in the G93A mouse, an animal model of ALS.PLoS One. 2010 Feb 24;5(2):e9386. doi: 10.1371/journal.pone.0009386. PLoS One. 2010. PMID: 20195368 Free PMC article.
-
Resveratrol supplementation at old age reverts changes associated with aging in inflammatory, oxidative and apoptotic markers in rat heart.Eur J Nutr. 2021 Aug;60(5):2683-2693. doi: 10.1007/s00394-020-02457-0. Epub 2021 Jan 2. Eur J Nutr. 2021. PMID: 33386891
-
Dose-dependent effects of calorie restriction on gene expression, metabolism, and tumor progression are partially mediated by insulin-like growth factor-1.Cancer Med. 2012 Oct;1(2):275-88. doi: 10.1002/cam4.23. Epub 2012 Aug 6. Cancer Med. 2012. PMID: 23342276 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Research Materials