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. 2003 Aug;9(8):1679-82.
doi: 10.3748/wjg.v9.i8.1679.

Assessment of hepatic VX2 tumors of rabbits with second harmonic imaging under high and low acoustic pressures

Wen-Hua Du  1 , Wei-Xiao Yang, Xiang Wang, Xiu-Qin Xiong, Yi Zhou, Tao Li
Affiliations

Assessment of hepatic VX2 tumors of rabbits with second harmonic imaging under high and low acoustic pressures

Wen-Hua Du et al. World J Gastroenterol. 2003 Aug.

Abstract

Aim: To investigate the possible clinical application value of second harmonic imaging under low acoustic pressure.

Methods: Six New Zealand rabbits, averaging 2.7+/-0.4 kg, were selected and operated upon to construct hepatic VX2 tumor carrier model. Hepatic VX2 tumors were imaged with B mode Ultrasonography (US), and second harmonic imaging (SHI) under high mechanic index (1.6) and low mechanic index (0.1). Echo agent was intravenously injected through ear vein at a dose of 0.01 mL/kg under B mode US and high MI SHI, and 0.05 mL/kg under low MI SHI, and then the venous channel was cleaned with sterilized saline. All the images were recorded by magnetic optics (MO), and they were analyzed further by at least two independent experienced sonographers.

Results: Totally 6 hypoechoic and 3 hyperechoic lesions were found in the six carrier rabbits with a mean size about 2.1+/-0.4 under B mode ultrasound, they were oval or round in shape with a clear outline or a hypoechoic halo at the margin of the lesions. Contrast agent could not change the echogenicity of the lesions under B mode US and SHI under high acoustic pressure. However, it could greatly increase the real time visualization sensitivity of the lesions with SHI under low acoustic pressure.

Conclusion: Our results suggest that contrast enhanced SHI with low MI and a bubble non-destructive method would be much more helpful than conventional SHI in our future clinical applications.

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Figures

Figure 1
Figure 1
Image of VX2 tumor lesion under conventional B mode US. Arrow indicated the VX2 tumor lesion at the anterior part of the right lobe. It was oval and hypoechoic with a small hyperechoic scar at the center of the lesion.
Figure 2
Figure 2
a: Image of VX2 tumor lesion under high MI second harmonic imaging. Arrow indicated the nutrient artery of same VX2 tumor lesion, and the suspicious artery of a satellite lesion. b: Contrast enhanced second harmonic image under low MI revealed the enhancement of nutrient arteries of VX2 tumor lesion and the nutritive artery of satellite lesion.
Figure 3
Figure 3
Image of second harmonic under low MI. It showed the clear tumor and its satellite lesion.
See this image and copyright information in PMC

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