Characterization of a wound-inducible cytochrome P450 gene ( CYP72A29) that is down-regulated during crown gall tumorigenesis in potato tuber

Abstract

A cDNA for a putative cytochrome P450 (CYP72A29), which is down-regulated during Agrobacterium tumefaciens -mediated tumorigenesis, was isolated from potato ( Solanum tuberosumL. May Queen) tuber by differential display. Northern analysis indicated that the CYP72A29 gene was transiently up-regulated in tuber discs after a 24-h aging period, and expression gradually increased upon further incubation (up to 7 days). Inoculation of tuber discs with the non-pathogenic A. tumefaciens strain LBA4301 had a very similar effect, but inoculation with the wild-type strain A. tumefaciens A208 suppressed the accumulation of mRNA during further incubation. Furthermore, the accumulation of CYP72A29 mRNA was strongly suppressed by inoculation with mutant strains of A. tumefaciens that produce large amounts of indole-3-acetic acid. This down-regulation also occurred when the discs were treated with 2,4-dichlorophenoxyacetic acid or 1-naphthalenacetic acid. These results suggest that the expression of CYP72A29 is regulated by auxin. RT-PCR analysis revealed that the transcripts were most abundant in sprouts and eyes, less so in roots, and hardly detectable in leaves, flower buds and stems.