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Case Reports
. 2003 Sep;87(9):1190-1.
doi: 10.1136/bjo.87.9.1190.

Propionibacterium acnes endophthalmitis diagnosed by microdissection and PCR

R R Buggage, D G Callanan, D F Shen, C C Chan
Case Reports

Propionibacterium acnes endophthalmitis diagnosed by microdissection and PCR

R R Buggage et al. Br J Ophthalmol. 2003 Sep.

Erratum in

  • Br J Ophthalmol. 2003 Nov;87(11):1432
No abstract available

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Figures

Figure 1
Figure 1
(A) Photomicrograph of the vitreous specimen showing clusters of macrophages admixed with T and B lymphocytes. Many degenerated lymphocytes were also present. (Giemsa, ×200). (B) Higher power photomicrograph of the vitreous specimen showing Gram positive pleomorphic bacilli (arrow) in the cytoplasm of a few macrophages (Gram stain, ×640).
Figure 1
Figure 1
(A) Photomicrograph of the vitreous specimen showing clusters of macrophages admixed with T and B lymphocytes. Many degenerated lymphocytes were also present. (Giemsa, ×200). (B) Higher power photomicrograph of the vitreous specimen showing Gram positive pleomorphic bacilli (arrow) in the cytoplasm of a few macrophages (Gram stain, ×640).
Figure 2
Figure 2
Nested PCR products of the microdissected bacilli from the vitreous specimen. The first round used Pa1 and rPa2. The second round used Pa1 and rPa3. The negative control in both rounds contained no DNA. The positive control in both rounds was P acnes.
See this image and copyright information in PMC

References

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