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Comparative Study
. 2003 Sep;71(9):5402-6.
doi: 10.1128/IAI.71.9.5402-5406.2003.

Genetic variation influences the B-cell response to immunization with a pneumococcal polysaccharide conjugate vaccine

T L McCool  1 , J R Schreiber, N S Greenspan
Affiliations
Comparative Study

Genetic variation influences the B-cell response to immunization with a pneumococcal polysaccharide conjugate vaccine

T L McCool et al. Infect Immun. 2003 Sep.

Abstract

CBA/J mice immunized with pneumococcal 23F-CRM(197) vaccine produce significantly lower titers of 23F-specific antibodies and fewer 23F-specific antibody-secreting cells (ASC) than did BALB/c or (CBA/J x BALB/c)F(1) (CCBAF(1)) mice. The reduced 23F-specific titers of CBA/J versus BALB/c or CCBAF(1) mice are presumably related to lower frequencies of 23F-specific ASC influenced by genetic variation.

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Figures

FIG. 1.
FIG. 1.
23F- and CRM197-specific responses of BALB/c, CBA/J, and CCBAF1 mice after two immunizations with 23F-CRM197. (A) Total 23F-specific serum immunoglobulin κ chain titers (micrograms per milliliter) detected by PnPS solid-phase ELISA. (B) Frequency of 23F-specific κ ASC determined by PnPS solid-phase ELISpot assay. The data shown are a compilation of two experiments with the mean ± the standard error of the mean of a total of 24 mice per group. *, P < 0.004. (C)Total CRM197-specific serum immunoglobulin κ chain titers (micrograms per milliliter) detected by CRM197 solid-phase ELISA. (D) Frequency of CRM197-specific κ ASC determined by PnPS solid-phase ELISpot assay. The data shown are a compilation of two experiments with the mean ± the standard error of the mean of a total of 24 mice per group. *, P < 0.003 for comparisons of selected groups with CCBAF1.
FIG. 2.
FIG. 2.
19F- and CRM197-specific responses of BALB/c, CBA/J, and CCBAF1 mice after two immunizations with 19F-CRM197. (A) Total 19F-specific serum immunoglobulin κ chain titers (micrograms per milliliter) detected by PnPS solid-phase ELISA. (B) Frequency of 19F-specific κ ASC determined by PnPS solid-phase ELISpot assay. The data shown are a compilation of two experiments with the mean ± the standard error of the mean of a total of 16 mice per group. (C) Total CRM197-specific serum immunoglobulin κ chain titers (micrograms per milliliter) detected by CRM197 solid-phase ELISA. (D) Frequency of CRM197-specific κ ASC determined by CRM197 solid-phase ELISpot assay. The data shown are a compilation of two experiments with the mean ± the standard error of the mean of a total of 16 mice per group.
FIG. 3.
FIG. 3.
PS- and CRM197- specific antibody titers (micrograms per milliliter) of CBA/J mice primed with either 19F-CRM197 or 23F-CRM197 and boosted with either conjugate. Total serum immunoglobulin κ chain titers (micrograms per milliliter) of 19F-specific (A), 23F-specific (B), and CRM197-specific (C) antibodies in CBA/J mice detected by PnPS (A and B) and CRM197 (C and D) solid-phase ELISAs. 19F-specific (A) and CRM197-specific (D) IgG1 levels detected in the same sera are also shown. The mean titers (micrograms per milliliter) ± the standard error of the mean of groups of five mice are shown.
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References

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