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. 2003;27(6):326-30.
doi: 10.1007/s00264-003-0496-z. Epub 2003 Aug 26.

Compressed homologous cancellous bone and bone morphogenetic protein (BMP)-7 or bone marrow accelerate healing of long-bone critical defects

T Djapic  1 , V Kusec, M Jelic, S Vukicevic, M Pecina
Affiliations

Compressed homologous cancellous bone and bone morphogenetic protein (BMP)-7 or bone marrow accelerate healing of long-bone critical defects

T Djapic et al. Int Orthop. 2003.

Abstract
in English, French

We studied 18 adult male New Zealand rabbits with a critical right-sided ulnar defect of 15 mm. In six animals the defect was grafted with homologous compressed cancellous bone, in six animals with homologous compressed cancellous bone including 300 micro g bone morphogenetic protein (BMP)-7 and in six animals with homologous compressed cancellous bone including 0.5 ml autologous bone marrow. The defect was studied using radiographs every second week for 10 weeks. At the conclusion of the experiment the animals were killed and the defect studied by histology and histomorphometry. In all animals treated with the addition of autologous bone marrow and in five of six animals treated with the addition of BMP-7, the defect healed. There was no union in animals treated with homologous compressed cancellous bone without additive. The histological picture of the regenerated area was similar in the two experimental groups. Woven bone contained small marrow spaces with fibrous tissue and capillaries. The osteoid seams were on average greater in animals that received autologous bone marrow as compared to animals that received BMP-7.

Nous avons étudié 18 lapins mâles adultes de Nouvelle Zélande avec une perte de substance ulnaire droite de 15 mm. Chez six animaux le défaut a été greffé avec de l'os spongieux compressé homologue, chez six autres avec de l'os spongieux compressé homologue incluant 300 micrograms de BMP-7 et chez les six animaux restant, avec de l'os spongieux compressé homologue incluant 0,5 ml de moelle osseuse autologue. Le défaut osseux a été étudié par des radiographies toutes les 2 semaines pendant dix semaines. À la fin de l'expérience les animaux ont été sacrifiés et le défaut a été étudié par histologie et par histomorphométrie. Chez tous les animaux traités avec addition de moelle osseuse autologue et chez cinq des six animaux traités avec addition de BMP-7 le défaut a consolidé. Il n'y avait aucune consolidation chez les animaux traités avec de l'os spongieux compressé homologue sans additif. Les images histologiques de la région régénérée était semblable dans les deux groupes expérimentaux. L'os nouveau contenait des petits espaces médullaires avec tissu fibreux et capillaires. La bordure ostéoide était en moyenne plus grande chez les animaux qui avaient reçu de la moelle autologue comparés à ceux qui avaient reçu de la BMP-7.

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Figures

Fig. 1A–D.
Fig. 1A–D.
A compressed homologous cancellous radiodense bone cylinder without supplement fills the gap immediately after surgery (A), but very little material remains after 10 weeks (B). At ten weeks rabbits treated with compressed homologous cancellous bone with autologous bone marrow (C) or BMP-7 (D) showed complete healing with bone bridging
Fig. 2. A
Fig. 2. A
Specimen from rabbit treated with compressed cancellous bone without supplement. The gap was filled with fibrous and adipose tissue, prolapsed muscle and pieces of devitalised bone (DB). Bone fragments (BF) were undergoing intensive remodelling (×40, toluidine blue). (B) Specimen from rabbit treated with compressed cancellous bone and bone marrow. New bone (NB) formed in the ulnar defect contained bone marrow spaces (BMS) with dense fibrous tissue and capillaries (×40, toluidine blue). (C) Specimen from rabbit treated with compressed cancellous bone and BMP-7. Bone marrow spaces (BMS) were filled with fibrous tissue in the new bone (NB) formed in the defect in (40×, toluidine blue). (D) Thickness of the osteoid seams in the regenerate area differed between the treatment groups (P=0.03). 2: Control group (homologous compressed cancellous bone). 3: Group treated with homologous compressed cancellous bone and autologous bone marrow. 4: Group treated with homologous compressed cancellous bone and BMP-7
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