Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2003 Sep;56(9):699-702.
doi: 10.1136/jcp.56.9.699.

Diffuse and intestinal type gastric carcinomas differ in their expression of apoptosis related proteins

C J van der Woude  1 , J H Kleibeuker, A T G M Tiebosch, M Homan, A Beuving, P L M Jansen, H Moshage
Affiliations
Comparative Study

Diffuse and intestinal type gastric carcinomas differ in their expression of apoptosis related proteins

C J van der Woude et al. J Clin Pathol. 2003 Sep.

Abstract

Background: Gastric carcinomas can be divided into intestinal and diffuse types, with the last type having a worse prognosis.

Aims: To investigate whether specific patterns in the expression of apoptosis related proteins correlate with carcinoma type and/or prognosis

Methods: The expression of Fas, Bcl-2, Bax, Bcl-xl, cyclooxygenase-2 (COX-2), and inducible nitric oxide synthase (iNOS) was studied immunohistochemically and the extent of apoptosis and proliferation was investigated in 11 cases of intestinal type and in eight cases of diffuse type carcinoma.

Results: Fas was expressed in all intestinal type and in one diffuse type carcinoma. Bcl-xl was expressed in 10 of 11 intestinal type and in one of eight diffuse type carcinomas. Bcl-2 was expressed in lamina propria immune cells. iNOS was expressed in six of 11 intestinal type and in four of eight diffuse type carcinomas, and COX-2 was expressed in eight of 11 intestinal type and in six of eight diffuse type carcinomas.

Conclusion: Fas and Bcl-xl expression can differentiate between intestinal type and diffuse type gastric carcinomas. No differences in apoptosis and proliferation between intestinal type and diffuse type gastric carcinomas were observed.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Fas immunohistochemistry in (A) intestinal and (B) diffuse type gastric cancer. Bcl-xl immunohistochemistry in (C) intestinal and (D) diffuse type gastric cancer. Note the positive staining for Fas (A) and Bcl-xl (C) in the intestinal type gastric cancer. Only one of seven diffuse type carcinomas stained positively for Fas (the sample shown here is Fas negative; B). In addition, only one of seven diffuse type carcinomas stained positively for Bcl-xl (the sample shown here is Bcl-xl negative; D).
Figure 2
Figure 2
Example of active caspase 3 staining in a diffuse type carcinoma. Note that not all tumour cells were positive for active caspase 3.
See this image and copyright information in PMC

References

    1. Lauren P. The two histological main types of gastric cancer: diffuse and so-called intestinal type carcinoma. Acta Pathol Microbiol Scand 1965;64:331–49. - PubMed
    1. Sipponen P, Kekki M, Siurala M. Atrophic chronic gastritis and intestinal metaplasia in gastric carcinoma. Cancer 1983;52:1062–8. - PubMed
    1. Adachi Y, Yasuda K, Inomata M, et al. Pathology and prognosis of gastric carcinoma. Cancer 2000;89:1418–24. - PubMed
    1. Waring P, Mullbacher A. Cell death induced by the Fas/Fas ligand pathway and its role in pathology. Immunol Cell Biol 1999;77:312–17. - PubMed
    1. Shinohara T, Ohshima K, Murayama H, et al. Apoptosis and proliferation in gastric carcinoma: the association with histological type. Histopathology 1996;29:123–9. - PubMed

Publication types

MeSH terms