[Subclasses of serum HDL in hyperlipidemia]

Abstract

Objective: To detect the change of composition and ratio of serum HDL subclasses and explore the relationship between these changes and the plasma lipid level in patients with hyperlipidemia.

Methods: The subclasses of serum HDL in 168 patients with hyperlipidemia were determined by two-dimensional gel electrophoresis in conjunction with immunodetection method.

Results: Compared to the healthy controls, the pre-beta 1 HDL, pre-beta 2 HDL, HDL3c, HDL3b, and HDL3a increased significantly (P < 0.01, P < 0.01, P < 0.05, P < 0.01 and P < 0.01), while the HDL2a and HDL2b decreased significantly (P < 0.01) in the patients with hyperlipidemia. The pre-beta 1 HDL and HDL3b were significantly higher (P < 0.01) while the HDL2a and HDL2b were significantly lower (P < 0.01) in men than in women with hyperlipidemia. The pre-beta 1 HDL was significantly higher (P < 0.05) while the HDL2b was significantly lower (P < 0.05) in the male control group than in the female control group. In the patients with hyperlipidemia, the serum TG was positively correlated with pre-beta 1 HDL, pre-beta 2 HDL, HDL3b, and HDL3a (r = 0.583, 0.196, 0.130, and 0.370 respectively) and negatively correlated with HDL2a and HDL2b (r = -0.293 and -0.456 respectively); The serum TC was positively correlated with pre-beta 1 HDL and HDL3b (r = 0.348 and 0.219); The serum LDL-C was positively correlated with HDL3b (r = 0.187); The serum HDL-C was negatively correlated with pre-beta 1 HDL and HDL3a (r = -0.354 and -0.292 respectively) and positively correlated with HDL2a and HDL2b (r = 0.254 and 0.256); The TG/HDL-C ratio was positively correlated with pre-beta 1 HDL, pre-beta 2 HDL, HDL3b, and HDL3a (r = 0.593, 0.225, 0.123, and 0.394) and negatively correlated with HDL2a and HDL2b (r = -0.317 and -0.459).

Conclusion: The particle of HDL in the patients with hyperlipidemia shows a general shift towards smaller size, which indicates that the maturation of HDL could be abnormal in patients with hyperlipidemia. The change of subclasses of HDL in the patients with hyperlipidemia might be associated with coronary artery diseases.