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. 2003 Sep;41(9):4418-20.
doi: 10.1128/JCM.41.9.4418-4420.2003.

Evaluation of INNO-LiPA MYCOBACTERIA v2: improved reverse hybridization multiple DNA probe assay for mycobacterial identification

Enrico Tortoli  1 , Alessandro Mariottini, Gianna Mazzarelli
Affiliations

Evaluation of INNO-LiPA MYCOBACTERIA v2: improved reverse hybridization multiple DNA probe assay for mycobacterial identification

Enrico Tortoli et al. J Clin Microbiol. 2003 Sep.

Abstract

INNO-LiPA MYCOBACTERIA (Innogenetics, Ghent, Belgium) is a reverse hybridization DNA probe assay that has been recently improved by increasing the number of identifiable mycobacterial species to 16. Our assessment, performed with 197 mycobacteria belonging to 81 taxa, revealed 100% specificity and sensitivity for 20 out of 23 probes. The probes specific for Mycobacterium fortuitum complex, for the Mycobacterium avium-intracellulare-scrofulaceum group, and for Mycobacterium intracellulare type 2 cross-reacted with several mycobacteria rarely isolated from clinical specimens. The overall sensitivity was 100%, and the overall specificity was 94.4%.

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References

    1. Cage, G. D. 1992. High-performance liquid chromatography patterns of Mycobacterium gordonae mycolic acids. J. Clin. Microbiol. 30:2402-2407. - PMC - PubMed
    1. Centers for Disease Control and Prevention. 1996. Standardized method for HPLC identification of mycobacteria. Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Atlanta, Ga. - PubMed
    1. Frothingham, R., and K. H. Wilson. 1993. Sequence-based differentiation of strains in the Mycobacterium avium complex. J. Bacteriol. 175:2818-2825. - PMC - PubMed
    1. Kirschner, P., and E. C. Böttger. 1992. Microheterogeneity within rRNA of Mycobacterium gordonae. J. Clin. Microbiol. 30:1049-1050. - PMC - PubMed
    1. Kirschner, P., B. Springer, U. Vogel, A. Meier, A. Wrede, M. Kiekenbeck, F. C. Bange, and E. C. Böttger. 1993. Genotypic identification of mycobacteria by nucleic acid sequence determination: report of a 2-year experience in a clinical laboratory. J. Clin. Microbiol. 31:2882-2889. - PMC - PubMed

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