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Case Reports
. 2003 Sep;41(9):4460-1.
doi: 10.1128/JCM.41.9.4460-4461.2003.

Shell vial culture as a tool for isolation of Brucella melitensis in chronic hepatic abscess

Clarisse Rovery  1 , Jean Marc Rolain, Didier Raoult, Philippe Brouqui
Affiliations
Case Reports

Shell vial culture as a tool for isolation of Brucella melitensis in chronic hepatic abscess

Clarisse Rovery et al. J Clin Microbiol. 2003 Sep.

Abstract

A 50-year-old man was admitted with a liver abscess and positive serology for Brucella spp. Liver pus and blood cultures remained sterile on conventional culture. Inoculation of liver pus onto eukaryotic cells by the centrifugation-shell vial technique yielded Brucella melitensis, identified by 16S rRNA gene amplification and sequencing.

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Figures

FIG. 1.
FIG. 1.
Gimenez staining of a coverslip from a shell vial after 21 days of incubation showing small extracellular and apparently intracellular coccobacilli within HEL cells. Magnification, ×1,000.
FIG. 2.
FIG. 2.
Immunofluorescence staining of the HEL cell monolayer by using the patient's serum as the primary antibody. Magnification, ×400.
See this image and copyright information in PMC

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