Nicotinamide induces both proliferation and differentiation of embryonic stem cells into insulin-producing cells

Abstract

In contrast to the consistent observation that methods that promote proliferation also dedifferentiate insulin-producing cells, useful in vitro differentiation protocols must drive both proliferation and differentiation. We herein describe an strategy in which the combination of nutrient restriction and nicotinamide supplementation results in a consistent increase in the mass of insulin-producing cells.