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. 1976 Dec 11;71(2):443-9.
doi: 10.1111/j.1432-1033.1976.tb11132.x.

Interaction of DNA with DNA-binding proteins. The characterization of protein HD from Escherichia coli and its nucleic acid complexes

V Berthold, K Geider
Free article

Interaction of DNA with DNA-binding proteins. The characterization of protein HD from Escherichia coli and its nucleic acid complexes

V Berthold et al. Eur J Biochem. .
Free article

Abstract

DNA-binding protein HD with a monomer molecular weight of 9000 was isolated from Escherichia coli cells. The protein occurs as a tetramer under native conditions and binds to single and double-stranded DNA and also to RNA. DNA complexed with protein HD is a poor template for DNA synthesis by E. coli polymerase I, II or III holoenzyme. Exonuclease III is hindered in degrading HD-protein-covered double-stranded DNA, whereas exonuclease I can digest complexed single-stranded DNA. Transcription is liqhtly stimulated in the presence of protein HD.

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