[Identification of the causative agents of glanders and melioidosis by polymerase chain reaction]
[Article in
Russian]
Affiliations
- PMID: 12966921
Item in Clipboard
[Identification of the causative agents of glanders and melioidosis by polymerase chain reaction]
[Article in
Russian]
Mol Gen Mikrobiol Virusol.
2003.
Abstract
Burkholderia mallei and B. pseudomallei are causative agents of glanders and melioidosis, respectively, i.e. severe and fatal infection diseases of man and animal. The computer-based analysis of the 23S rRNA gene sites was used for selecting the primers. Two pairs of primers were chosen for the identification of B. mallei and Bpseudomallei. DNAs from 48 B. pseudomallei and 15 strains of B. mallei, unlike from other geterological bacteria, were positively amplified. Therefore, the method of polymerase chain reaction can be used in laboratory diagnosis of glanders and melioidosis.
Similar articles
-
[Molecular-genetic approaches to diagnosis and intraspecific typing of causative agents of glanders and melioidosis].Mol Gen Mikrobiol Virusol. 2005;(2):3-9. Mol Gen Mikrobiol Virusol. 2005. PMID: 15954468 Review. Russian.
-
Development of a polymerase chain reaction assay for the specific identification of Burkholderia mallei and differentiation from Burkholderia pseudomallei and other closely related Burkholderiaceae.Diagn Microbiol Infect Dis. 2006 May;55(1):37-45. doi: 10.1016/j.diagmicrobio.2005.11.007. Epub 2006 Mar 20. Diagn Microbiol Infect Dis. 2006. PMID: 16546342
-
Identification and discrimination of Burkholderia pseudomallei, B. mallei, and B. thailandensis by real-time PCR targeting type III secretion system genes.J Clin Microbiol. 2004 Dec;42(12):5871-4. doi: 10.1128/JCM.42.12.5871-5874.2004. J Clin Microbiol. 2004. PMID: 15583328 Free PMC article.
-
Detection and differentiation of Burkholderia pseudomallei, Burkholderia mallei and Burkholderia thailandensis by multiplex PCR.FEMS Immunol Med Microbiol. 2005 Mar 1;43(3):413-7. doi: 10.1016/j.femsim.2004.10.008. FEMS Immunol Med Microbiol. 2005. PMID: 15708316
-
PCR-based Methodologies Used to Detect and Differentiate the Burkholderia pseudomallei complex: B. pseudomallei, B. mallei, and B. thailandensis.Curr Issues Mol Biol. 2014;16:23-54. Epub 2013 Aug 22. Curr Issues Mol Biol. 2014. PMID: 23969318 Review.