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Comparative Study
. 2003 Jul;107(Pt 7):803-10.
doi: 10.1017/s0953756203008086.

Agrobacterium and PEG-mediated transformation of the phytopathogen Venturia inaequalis

Anna M Fitzgerald  1 , Agnieszka M Mudge, Andrew P Gleave, Kim M Plummer
Affiliations
Comparative Study

Agrobacterium and PEG-mediated transformation of the phytopathogen Venturia inaequalis

Anna M Fitzgerald et al. Mycol Res. 2003 Jul.

Abstract

We report the development of two new transformation systems, polyethylene glycol (PEG)-mediated transformation of protoplasts and Agrobacterium tumefaciens-mediated transformation of mycelium, for the filamentous ascomycete Venturia inaequalis. New binary vectors have been created for the latter. Although transformation was initially achieved using a PEG-mediated method, this was superseded by the A. tumefaciens-mediated approach. The advantages of the latter include: ease of the protocol, no requirement for protoplasts; higher transformation efficiency; and single-site integration. A comparison between the two transformation methods is presented.

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