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. 2003 Sep 12;301(5639):1545-7.
doi: 10.1126/science.1087117.

Transport of dsRNA into cells by the transmembrane protein SID-1

Evan H Feinberg  1 , Craig P Hunter
Affiliations

Transport of dsRNA into cells by the transmembrane protein SID-1

Evan H Feinberg et al. Science. .

Abstract

RNA interference (RNAi) spreads systemically in plants and nematodes to silence gene expression distant from the site of initiation. We previously identified a gene, sid-1, essential for systemic but not cell-autonomous RNAi in Caenorhabditis elegans. Here, we demonstrate that SID-1 is a multispan transmembrane protein that sensitizes Drosophila cells to soaking RNAi with a potency that is dependent on double-stranded RNA (dsRNA) length. Further analyses revealed that SID-1 enables passive cellular uptake of dsRNA. These data indicate that systemic RNAi in C. elegans involves SID-1-mediated intercellular transport of dsRNA.

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