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. 2003 Sep;9(9):2009-11.
doi: 10.3748/wjg.v9.i9.2009.

Abnormal immunity and gene mutation in patients with severe hepatitis-B

Jing-Yan Wang  1 , Pei Liu
Affiliations

Abnormal immunity and gene mutation in patients with severe hepatitis-B

Jing-Yan Wang et al. World J Gastroenterol. 2003 Sep.

Abstract

Aim: To evaluate the abnormal immunity and gene mutation at precore 1896 site in patients with severe hepatitis-B.

Methods: This study included 23 patients with severe hepatitis-B, 22 patients with acute hepatitis-B and 20 controls. Mutation at precore 1896 site of HBV gene was confirmed with restriction fragment length polymorphism (RFLP) analysis. Cytokines including TNF-alpha, IFN-gamma, IL-6, and IL-8 were measured with ELISA, and T subgroups were detected with alkaline phosphatase anti alkaline phosphatase (APAAP) technique.

Results: In patients with severe hepatitis-B, the infective rate of HBV mutant strain was 52.5 % (12/23), and only one patient with acute hepatitis-B was infected with the mutant strain. The percentage of CD8+ T lymphocyte was obviously lower (0.16+/-0.02 %) and the ratio of CD4+/CD8+ was obviously higher (2.35+/-0.89) in mutant group than in wild-type group (0.28+/-0.05 % and 1.31+/-0.18 %, respectively, P<0.01 or P<0.05). The levels of cytokines in patients with severe hepatitis-B were higher (TNF-alpha 359.0+/-17.2 ng/L, IFN-gamma 234.7+/-16.5 ng/L, IL-6 347.5+/-31.3 ng/L, IL-8 181.1+/-19.6 ng/L) than those in acute hepatitis-B (TNF-alpha 220.6+/-8.9 ng/L, IFN-gamma 174.9+/-12.0 ng/L, IL-6 285.8+/-16.5 ng/L, IL-8 118.4+/-5.1 ng/L, P<0.01 or 0.05). In patients with severe hepatitis-B, the levels of IFN-gamma and IL-6 were higher in mutant group (273.4+/-26.6 ng/L, 387.7+/-32.5 ng/L) than in wild-type group (207.8+/-12.8 ng/L, 300.9+/-16.3 ng/L). The mortality of patients infected with HBV mutant strain was higher (100 %) than that with wild-type (0.9 %).

Conclusion: In severe hepatitis-B, the infective rate of HBV mutant strain was high. The mutant strain induces more severe immune disorders in host, resulting in the activation of lymphocyte and release of cytokines. HBV DNA mutates easily in response to the altered immunity. Ultimately liver damage is more prominent.

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References

    1. Niitsuma H, Ishii M, Miura M, Toyota T. [Detection of HBV precore mutation by PCR-RFLP] Nihon Rinsho. 1995;53 Suppl:316–320. - PubMed
    1. Chu CM. Natural history of chronic hepatitis B virus infection in adults with emphasis on the occurrence of cirrhosis and hepatocellular carcinoma. J Gastroenterol Hepatol. 2000;15 Suppl:E25–E30. - PubMed
    1. Bertoletti A, Sette A, Chisari FV, Penna A, Levrero M, De Carli M, Fiaccadori F, Ferrari C. Natural variants of cytotoxic epitopes are T-cell receptor antagonists for antiviral cytotoxic T cells. Nature. 1994;369:407–410. - PubMed
    1. Ohta A, Sekimoto M, Sato M, Koda T, Nishimura S, Iwakura Y, Sekikawa K, Nishimura T. Indispensable role for TNF-alpha and IFN-gamma at the effector phase of liver injury mediated by Th1 cells specific to hepatitis B virus surface antigen. J Immunol. 2000;165:956–961. - PubMed
    1. Muto Y, Nouri-Aria KT, Meager A, Alexander GJ, Eddleston AL, Williams R. Enhanced tumour necrosis factor and interleukin-1 in fulminant hepatic failure. Lancet. 1988;2:72–74. - PubMed

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