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. 2003 Sep;41(3):155-63.
doi: 10.3347/kjp.2003.41.3.155.

Specific and common antigens of Clonorchis sinensis and Opisthorchis viverrini(Opisthorchidae, Trematoda)

Min-Ho Choi  1 , Jin-Sook Ryu, Mejeong Lee, Shunyu Li, Byung-Suk Chung, Jong-Yil Chai, Paiboon Sithithaworn, Smarn Tesana, Sung-Tae Hong
Affiliations

Specific and common antigens of Clonorchis sinensis and Opisthorchis viverrini(Opisthorchidae, Trematoda)

Min-Ho Choi et al. Korean J Parasitol. 2003 Sep.

Abstract

The antigenic characterizations and serological reactions of human liver flukes, Clonorchis sinensis and Opisthorchis viverrini, were analyzed by immunoblot. The antigenic profiles of the crude extract of Clonorchis contained major proteins of 8, 26-28, 34-37, 43, and 70 kDa, and those of Opisthorchis 34-37, 43, 70, and 100 kDa. Of these, the 8, 26-28 and 34-37 kDa bands of Clonorchis and the 100 kDa of Opisthorchis were major components of each excretory-secretory antigen. The 8 and 26-28 kDa bands were specific to Clonorchis but the 100 kDa of Opisthorchis cross-reacted with the sera of clonorchiasis, and the 34-37, 70 and 100 kDa bands cross-reacted with sera of other helminthiases. The frequency and intensity of the immunoblot reactions were positively correlated with the intensity of the liver fluke infection.

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Figures

Fig. 1
Fig. 1
Soluble protein bands separated by SDS-PAGE of crude extracts and excretory-secretory (ES) proteins of Clonorchis sinensis (Cs) and Opisthorchis viverrini (Ov).
Fig. 2
Fig. 2
Immunoblot patterns of the crude and ES antigens of Clonorchis sinensis (Cs) and the crude antigen of Opisthorchis viverrini (Ov) with the sera of clonorchiasis or opisthorchiasis subjects.
Fig. 3
Fig. 3
Immunoblot patterns of crude antigens of Clonorchis sinensis with sera of clonorchiasis subjects of various EPG counts.
Fig. 4
Fig. 4
Cross-reactions of the crude or ES antigens of Clonorchis sinensis and the crude antigen of Opisthorchis viverrini with sera of other helminthiasis subjects.
See this image and copyright information in PMC

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