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Comparative Study
. 1992 Dec;23(4):740-4.

Two-site pan-species monoclonal antibody ELISA for detection of blood stage malaria antigen

Q Gao  1 , S Tharavanij, S Khusmith, L Q Wang, S Looareesuwan, W G Zhang
Affiliations
  • PMID: 1298083
Comparative Study

Two-site pan-species monoclonal antibody ELISA for detection of blood stage malaria antigen

Q Gao et al. Southeast Asian J Trop Med Public Health. 1992 Dec.

Erratum in

  • Southeast Asian J Trop Med Public Health 1993 Sep;24(3):613

Abstract

A two-site pan-species monoclonal antibody sandwich ELISA (MAb-MAb ELISA) was developed to detect both Plasmodium vivax and P. falciparum antigens in whole blood impregnated on filter paper. In this assay, the plates were coated with pan-species MAb 3F9 and another pan-species MAb M26-32 conjugated with alkaline phosphatase was used for detection of bound antigen. The sensitivity of this assay was 5, 10 and 10 parasites per 10(6) erythrocytes for cultured P. falciparum, patient-derived P. vivax and P. falciparum, respectively. The coincidence rates for this assay were 93% (92/99) with healthy individuals and 93% (42/45) with microscopically confirmed vivax malaria cases. After two weeks treatment, 77.7% (14/18) of vivax malaria were still positive by this assay but with diminished level of reactivities [corrected].

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