Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1992 Jan;174(1):324-6.
doi: 10.1128/jb.174.1.324-326.1992.

A directional, high-frequency chromosomal mobilization system for genetic mapping of Rhizobium meliloti

S Klein  1 , K Lohman, R Clover, G C Walker, E R Signer
Affiliations

A directional, high-frequency chromosomal mobilization system for genetic mapping of Rhizobium meliloti

S Klein et al. J Bacteriol. 1992 Jan.

Abstract

A system for mapping of the Rhizobium meliloti chromosome that utilizes transposon Tn5-Mob, which carries the mobilization site of IncP plasmid RP4 (R. Simon, Mol. Gen. Genet. 196:413-420, 1984), was developed. Insertions of Tn5-Mob that were located at particular sites on the R. meliloti chromosome were isolated and served as origins of high-frequency chromosomal transfer when IncP tra functions were provided in trans. This approach is, in principle, applicable to any gram-negative bacterium in which Tn5 can transpose and into which IncP plasmids can conjugate.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1977 May;74(5):2076-8 - PubMed
    1. J Bacteriol. 1990 May;172(5):2469-76 - PubMed
    1. Cell. 1989 Feb 24;56(4):661-72 - PubMed
    1. J Bacteriol. 1988 Sep;170(9):4239-48 - PubMed
    1. J Bacteriol. 1986 Jul;167(1):66-72 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources