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. 1992;122(3-4):383-90.
doi: 10.1007/BF01317200.

Expression of feline immunodeficiency virus gag gene in Escherichia coli

T Furuya  1 , A Hasegawa, M Saitoh, T Miyazawa, Y Tohya, M Hayami, E Takahashi, K Miki, T Mikami
Affiliations

Expression of feline immunodeficiency virus gag gene in Escherichia coli

T Furuya et al. Arch Virol. 1992.

Abstract

The gag gene of a Japanese feline immunodeficiency virus (FIV) isolate, designated as FIV TM 2, was expressed in Escherichia coli as a fusion protein with TrpE. Using this expressed protein, an enzyme-linked immunosorbent assay was developed for detection of antibodies to FIV gag protein in feline sera. With serum samples from a cat experimentally infected with FIV, it was demonstrated that the period of seroconversion detected by this method corresponded to that by Western blotting.

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