Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1992 Feb;174(4):1240-7.
doi: 10.1128/jb.174.4.1240-1247.1992.

Isolation and characterization of the Escherichia coli htrD gene, whose product is required for growth at high temperatures

Affiliations

Isolation and characterization of the Escherichia coli htrD gene, whose product is required for growth at high temperatures

J M Delaney et al. J Bacteriol. 1992 Feb.

Abstract

Those genes in Escherichia coli defined by mutations which result in an inability to grow at high temperatures are designated htr, indicating a high temperature requirement. A new htr mutant of E. coli was isolated and characterized and is designated htrD. The htrD gene has been mapped to 19.3 min on the E. coli chromosome. Insertional inactivation of htrD with a mini-Tn10 element resulted in a pleiotropic phenotype characterized by a severe inhibition of growth at 42 degrees C and decreased survival at 50 degrees C in rich media. Furthermore, htrD cells were sensitive to H2O2. Growth rate analysis revealed that htrD cells grow very slowly in minimal media supplemented with amino acids. This inhibitory effect has been traced to the presence of cysteine in the growth medium. Further studies indicated that the rate of cysteine transport is higher in htrD cells relative to the wild type. All of these results, taken together, indicate that the htrD gene product may be required for proper regulation of intracellular cysteine levels and that an increased rate of cysteine transport greatly affects the growth characteristics of E. coli.

PubMed Disclaimer

Similar articles

Cited by

References

    1. J Bacteriol. 1986 Dec;168(3):1059-65 - PubMed
    1. J Biol Chem. 1975 Sep 25;250(18):7324-31 - PubMed
    1. Anal Biochem. 1976 May 7;72:248-54 - PubMed
    1. J Mol Biol. 1976 Jul 5;104(3):541-55 - PubMed
    1. J Bacteriol. 1991 Jan;173(2):741-50 - PubMed

Publication types

MeSH terms

LinkOut - more resources