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Comparative Study
. 1992 Jan;231(2):332-6.
doi: 10.1007/BF00279808.

Cloning and nucleotide sequence of the Escherichia coli K-12 ppsA gene, encoding PEP synthase

M Niersbach  1 , F Kreuzaler, R H Geerse, P W Postma, H J Hirsch
Affiliations
Comparative Study

Cloning and nucleotide sequence of the Escherichia coli K-12 ppsA gene, encoding PEP synthase

M Niersbach et al. Mol Gen Genet. 1992 Jan.

Abstract

We have cloned and sequenced the Escherichia coli K-12 ppsA gene. The ppsA gene codes for PEP synthase, which converts pyruvate into phosphoenolpyruvate (PEP), an essential step in gluconeogenesis when pyruvate or lactate are used as a carbon source. The open reading frame consists of 792 amino acids and shows homology with other phosphohistidine-containing enzymes that catalyze the conversion between pyruvate and PEP. These enzymes include pyruvate, orthophosphate dikinases from plants and Bacteroides symbiosus and Enzyme I of the bacterial PEP:carbohydrate phosphotransferase system.

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