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. 1976 Feb;30(2):277-80.

Antigen in immune complex nephritis. V. Recovery and identification by gel precipitation

F Milgrom, W A Campbell, G A Andres

Antigen in immune complex nephritis. V. Recovery and identification by gel precipitation

F Milgrom et al. Immunology. 1976 Feb.

Abstract

A procedure is described for detection of antibodies and antigens in 'immune complex' nephritis. Kidney tissue is minced, washed and sealed in a well of an agarose or agar plate. Separation of immune complexes is achieved by electrophoresis at 56 degrees. Afterwards, antibody is readily detected in reaction with a proper anti-IgG serum. Antigen is identified by its corresponding antibody, the reaction with which is, in some instances, strengthened by counterimmunoelectrophoresis.

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