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Comparative Study
. 1992 Feb;11(2):611-8.
doi: 10.1002/j.1460-2075.1992.tb05093.x.

Immunoglobulin V gene replacement is caused by the intramolecular DNA deletion mechanism

S Usuda  1 , T Takemori, M Matsuoka, T Shirasawa, K Yoshida, A Mori, K Ishizaka, H Sakano
Affiliations
Comparative Study

Immunoglobulin V gene replacement is caused by the intramolecular DNA deletion mechanism

S Usuda et al. EMBO J. 1992 Feb.

Abstract

Circular DNA resulting from V gene replacement was studied with an A-MuLV transformed cell line containing ablts. This cell line undergoes V gene replacement at elevated temperatures in the immunoglobulin (Ig) heavy chain (H) gene. Examination of circular DNA revealed that a heptamer-related sequence (TACTGTG) within the coding region of VDJ was joined to the recombination signal sequence (RSS) of a germline VH segment. This provides direct evidence for a intramolecular DNA deletion mechanism for V gene replacement. In the pre-B cell line as well as in in vivo lymphocytes, unusual circular DNAs were found which were structurally similar to the V gene replacement circles. They represented excision products of the deletion type recombination between one complete RSS and a heptamer-like sequence in the Ig H region.

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