doi: 10.1016/0378-1097(92)90630-7.
NAD-dependent glutamate dehydrogenase from Pseudomonas aeruginosa is a membrane-bound enzyme
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- PMID: 1311277
- DOI: 10.1016/0378-1097(92)90630-7
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NAD-dependent glutamate dehydrogenase from Pseudomonas aeruginosa is a membrane-bound enzyme
FEMS Microbiol Lett.
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Abstract
Measurements of the deaminating activity of NAD-dependent glutamate dehydrogenase (NAD-GDH) in Pseudomonas aeruginosa strain 8602 (PAC 1) showed an initially constant rate that gave way to a 3.5-fold increased rate on prolonged incubation. Only the faster rate was observed when assay mixtures were preflushed with nitrogen or were treated with the detergent Triton X-100. Comparison of the intracellular distribution of NAD-GDH with marker enzymes showed it to be associated with the cytoplasmic membrane. The results suggest that NAD-GDH may be linked to oxygen through an electron-transport system.
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