Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1992 Jan;83(1):66-71.
doi: 10.1111/j.1349-7006.1992.tb02353.x.

mRNA levels of catalytic subunits of protein phosphatases 1, 2A, and 2C in hepatocarcinogenesis

K Kitamura  1 , Y Mizuno, I Hatayama, K Sato, S Tamura, M Nagao, S Tsuiki, K Kikuchi
Affiliations

mRNA levels of catalytic subunits of protein phosphatases 1, 2A, and 2C in hepatocarcinogenesis

K Kitamura et al. Jpn J Cancer Res. 1992 Jan.

Abstract

The mRNA levels of three phosphoseryl/phosphothreonyl protein phosphatases, PP1, PP2A and PP2C, in rat liver have been determined by Northern blot analysis in various stages of rat chemical hepatocarcinogenesis using a Solt-Farber model. Five weeks after administration of diethylnitrosamine, the mRNA levels of PP1 alpha, PP2A and PP2C were elevated 8, 29 and 11 times, respectively, as compared to those of the control livers. However, in primary hepatoma induced according to the Solt-Farber model, the mRNA levels of all three protein phosphatases were dramatically decreased to normal levels or even to much lower levels, whereas the mRNA level of glutathione S-transferase placental form, a tumor marker protein, was greatly elevated as compared with that of the control livers. In a poorly differentiated hepatoma AH13, a line of rat ascites hepatoma, the mRNA level of PP1 alpha was 5.6 times higher than that of the control livers, whereas the mRNA lever of PP2C was almost the same as that of the control livers and the level of PP2A mRNA was distinctly lower than that of the control livers. These data appear to suggest some involvement of protein phosphatases in hepatocarcinogenesis.

PubMed Disclaimer

References

    1. ) Cohen , P.The structure and regulation of protein phosphatases . Annu. Rev. Biochem. , 58 , 453 – 508 ( 1989. ). - PubMed
    1. ) Hunter , T. and Cooper , A. J.Protein‐tyrosine kinases . Ann. Rev. Biochem. , 54 , 897 – 930 ( 1985. ). - PubMed
    1. ) Rosen , O.After insulin binds . Science , 237 , 1452 – 1458 ( 1987. ). - PubMed
    1. ) Yarden , Y. and Ullrich , A.Growth factor receptor tyrosine kinases . Ann. Rev. Biochem. , 57 , 443 – 478 ( 1988. ). - PubMed
    1. ) Bishop , M.J.Cellular oncogenes and retroviruses . Ann. Rev. Biochem. , 52 , 301 – 354 ( 1983. ). - PubMed

Publication types

MeSH terms

LinkOut - more resources