Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1992 Mar 23;300(1):71-2.
doi: 10.1016/0014-5793(92)80166-e.

Identification of a component separated on Mono Q purification of Escherichia coli RNA polymerase as an NTPase

J J Butzow  1 , R G Stankis
Affiliations
Free article

Identification of a component separated on Mono Q purification of Escherichia coli RNA polymerase as an NTPase

J J Butzow et al. FEBS Lett. .
Free article

Abstract

Standard preparations of Escherichia coli RNA polymerase (RNAP) contain NTPase activity. High-performance anion-exchange chromatography on Mono Q has recently been used by Hager et al. [1990, Biochemistry 29, 7890-7894] to fractionate RNAP into holoenzyme (alpha 2 beta beta' sigma) and core (alpha 2 beta beta') forms, plus other protein components. We found that one of these components, of protomer size slightly larger than the sigma 70 subunit, has NTPase activity; it is efficiently separated on Mono Q, leaving transcriptionally active holoenzyme and core apparently free of NTPase activity. Because of the similarity in size with sigma 70, the NTPase component may escape detection by routine gel electrophoresis.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

  • A structural model for fidelity in transcription.
    Eichhorn GL, Chuknyisky PP, Butzow JJ, Beal RB, Garland C, Janzen CP, Clark P, Tarien E. Eichhorn GL, et al. Proc Natl Acad Sci U S A. 1994 Aug 2;91(16):7613-7. doi: 10.1073/pnas.91.16.7613. Proc Natl Acad Sci U S A. 1994. PMID: 8052629 Free PMC article.

MeSH terms

LinkOut - more resources