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. 1992;123(1-2):223-8.
doi: 10.1007/BF01317152.

In vitro recognition of an orf virus early promoter in a vaccinia virus extract

J C Vos  1 , A A Mercer, S B Fleming, A J Robinson
Affiliations

In vitro recognition of an orf virus early promoter in a vaccinia virus extract

J C Vos et al. Arch Virol. 1992.

Abstract

DNA fragments containing varying lengths of the 5' end of an orf virus early gene (ORF3) and its associated promoter were introduced into sodium deoxycholate-solubilized vaccinia virus extracts capable of initiating transcription in vitro from vaccinia virus early promoters. After separation of the radiolabelled products of the reactions on a 5% polyacrylamide/7 M urea gel, discrete transcripts were detected the sizes of which were consistent with initiation of transcription from the orf virus early promoter. This is the first demonstration in a functional assay of the conservation of early transcriptional promoters between an orthopoxvirus and a parapoxvirus.

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