Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1992 Mar;30(3):577-80.
doi: 10.1128/jcm.30.3.577-580.1992.

Rapid method for epidemiological evaluation of gram-positive cocci by field inversion gel electrophoresis

R V Goering  1 , M A Winters
Affiliations

Rapid method for epidemiological evaluation of gram-positive cocci by field inversion gel electrophoresis

R V Goering et al. J Clin Microbiol. 1992 Mar.

Abstract

We report a rapid method for the isolation of intact chromosomal DNA from gram-positive cocci that is suitable for in situ restriction endonuclease digestion in agarose blocks. When combined with a rapid field inversion gel electrophoresis protocol, this approach allows the preparation and electrophoretic analysis of chromosomal restriction fragments produced by rare-cutting enzymes in a total time period of 2 days from start to finish. The utility of the method is demonstrated in the epidemiological evaluation of Staphylococcus epidermidis clusters from two hospitals as well as of additional representative staphylococci and enterococci.

PubMed Disclaimer

References

    1. J Infect Dis. 1990 Feb;161(2):230-5 - PubMed
    1. Infection. 1990 Jan-Feb;18(1):57-60 - PubMed
    1. J Clin Microbiol. 1991 Mar;29(3):648-9 - PubMed
    1. Biotechniques. 1990 Nov;9(5):542, 544, 546-50 - PubMed
    1. Biotechniques. 1989 Oct;7(9):938 - PubMed

Publication types

MeSH terms

LinkOut - more resources