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. 1992 Mar 25;20(6):1289-92.
doi: 10.1093/nar/20.6.1289.

Targeting deletion (homoeologous chromosome pairing locus) or addition line single copy sequences from cereal genomes

B Clarke  1 , P Stancombe, T Money, T Foote, G Moore
Affiliations
Free PMC article

Targeting deletion (homoeologous chromosome pairing locus) or addition line single copy sequences from cereal genomes

B Clarke et al. Nucleic Acids Res. .
Free PMC article

Abstract

We describe here a protocol for obtaining clones containing sequences present in low copy-number from genomic DNA where moderately and highly repeated sequences predominate. Specific chromosomal regions can be targeted by using deletion or addition line material. We have used this protocol to identify a sequence which has been deleted in both the tetraploid and hexaploid wheat mutants for the homoeologous chromosome pairing locus.

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