Cell swelling increases membrane conductance of canine cardiac cells: evidence for a volume-sensitive Cl channel

Abstract

Cardiac cell swelling occurs under abnormal conditions. Currents through volume-sensitive channels, if present in heart, will affect the cardiac electrical activity. Single canine ventricular myocytes were voltage clamped under conditions that largely suppressed Na, K, and Ca channel currents and currents generated by electrogenic transport systems. Cell width and membrane conductance were monitored continuously. Swelling was induced by increasing the osmolarity of the pipette solution or by decreasing the osmolarity of the external solution. During cell swelling, the cell widened and membrane conductance increased. This increase in membrane conductance was sensitive to Cl channel blockers and to external Cl removal, suggesting that a major component was provided by a Cl channel. The current-voltage relationship of the swelling-induced current displayed an outward rectification, with an average zero-current voltage of -60 mV. The activation of the swelling-induced current did not seem to depend on external or internal Ca and was not sensitive to a protein kinase inhibitor (H-8). Shape-altering agents chlorpromazine decreased while dipyridamole and trinitrophenol increased the membrane conductance without osmotic perturbations, suggesting that changes in tension in the cell membrane may play a role in opening and closing of the swelling-induced channels.