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. 1992 Jun;174(12):3928-35.
doi: 10.1128/jb.174.12.3928-3935.1992.

Cloning and nucleotide sequence of the leucyl-tRNA synthetase gene of Bacillus subtilis

P B Vander Horn  1 , S A Zahler
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Cloning and nucleotide sequence of the leucyl-tRNA synthetase gene of Bacillus subtilis

P B Vander Horn et al. J Bacteriol. 1992 Jun.

Abstract

The leucyl-tRNA synthetase gene (leuS) of Bacillus subtilis was cloned and sequenced. A mutation in the gene, leuS1, increases the transcription and expression of the ilv-leu operion, permitting monitoring of leuS alleles. The leuS1 mutation was mapped to 270 degrees on the chromosome. Sequence analysis showed that the mutation is a single-base substitution, possibly in a monocistronic operon. The leader mRNA predicted by the sequence would contain a number of possible secondary structures and a T box, a sequence observed upstream of leader mRNA terminators of Bacillus tRNA synthetases and the B. subtilis ilv-leu operon. The DNA of the B. subtilis leuS open reading frame is 48% identical to the leuS gene of Escherichia coli and is predicted to encode a polypeptide with 46% identity to the leucyl-tRNA synthetase of E. coli.

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